4',6-diamidino-2-phenylindole distinctly labels tau deposits
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چکیده
منابع مشابه
Use of the fluorochrome 4'6-diamidino-2-phenylindole in genetic and developmental studies of chloroplast DNA
Use of the DNA-specific fluorochrome 4'6-diamidino-2-phenylindole (DAPI) makes it possible to examine in situ the structure of chloroplast DNA (chDNA) with the fluorescence microscope. This simplifies the study of genetic and developmental changes in chloroplast DNA. Three examples are presented. (a) Wild-type Euglena gracilis B contains several chloroplast DNA nucleoids per chloroplast. A yell...
متن کاملFluorescence lifetime distributions of DNA-4',6-diamidino-2-phenylindole complex.
Time-resolved fluorescence of 4',6-diamidino-2-phenylindole (DAPI) complexes show that for a homogeneous polymer (polyd(AT) or polyd(A).polyd(T)) at high P/D (phosphate/dye) ratio, a single exponential component adequately describes the fluorescence decay. For the AT polymers at low P/D ratio or for native DNA, the decay cannot be described by a single-exponential term. A continuous distributio...
متن کامل1H NMR study of [d(GCGATCGC)]2 and its interaction with minor groove binding 4',6-diamidino-2-phenylindole.
Two-dimensional NMR spectroscopy has been applied to study the solution binding of 4',6-diamidino-2-phenylindole (DAPI) to synthetic DNA duplex [d(GCGATCGC)]2. The structure of the complex at a molar ratio of 1:1 drug:duplex has been investigated. NMR results indicate that DAPI binds selectively in the minor groove of the DNA region containing only two A:T base pairs. The results disagree with ...
متن کاملIn Higher Plants by M E a N S of the Dna - Specific Probe 4 ' 6 - Diamidino - 2 - Phenylindole
The D N A in isola ted chloroplas ts was visual ized by the f luorescent p robe 4 '6 d i amid ino-2 -pheny l indo le ( D A P I ) . When exci ted with light of 360 nm, the D N A D A P I complex f luoresces bri l l iant ly at 450 rim. Nucle i also f luoresce but their nucleoli do not . RNase and Pronase t r ea tmen t of chloroplas ts did not affect the f luorescence but both preand pos t t r ea t...
متن کاملVisualization by fluorescence of chloroplast DNA in higher plants by means of the DNA-specific probe 4'6-diamidino-2-phenylindole
The DNA in isolated chloroplasts was visualized by the fluorescent probe 4'6-diamidino-2-phenylindole (DAPI). When excited with light of 360 nm, the DNA-DAPI complex fluoresces brilliantly at 450 nm. Nuclei also fluoresce but their nucleoli do not. RNase and Pronase treatment of chloroplasts did not affect the fluorescence but both pre- and posttreatment of DAPI-stained chloroplasts with DNase ...
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ژورنال
عنوان ژورنال: Journal of the Neurological Sciences
سال: 2017
ISSN: 0022-510X
DOI: 10.1016/j.jns.2017.08.1906